1.Naturotics™?

Natural and Safe effective Preservative
Natural anti-bacterial, anti-fungal compound
Natural anti-oxidant complex and Effective humectant

2. Synthetic Preservatives & Naturotics™

Consumers who check labels often see parabens and other preservatives listed on a wide range of cosmetics and personal care products. As you know, Parabens and Isothiazolinone and its derivatives are chemicals used in cosmetics extensively and have bad effect on health hazardously. However, most people don’t realize that the hundreds of chemicals apply to their body every day; whether in the form of shampoo, conditioner, hand cream, face cream, shaving cream, makeup, and so on.

Nowadays people are trying to stay away from everything synthetic, including preservatives, as much as possible. This is due to increasing complications arising from the use of synthetic ingredients as carcinogenicity, teratogenicity, liver, kidney, heart, respiratory or nervous system problems.

Now, our unique knowledge of natural compound chemistry has resulted in Naturotics™. Naturotics™, a natural preservative, has been proved to be very effective against tested microorganisms at a concentration as low 2%. Also, Naturotics™ provides formulators of personal care products with any synthetic preservatives free solution which is supported by toxicology studies.

3. The main components of Naturotics™

4. EXPERIMENTAL DATA

(1) Challenge test

The results clearly demonstrated that Naturotics™ at 2.0% concentration reduced the microbial inoculums satisfying the E.P. in the skin toner and lotion formulation. Naturotics™ showed the ability to inhibit a variety of organisms including pathogenic organisms that may be introduced into the products.

(2) Anti-oxidant Activity

Everyday life, cell metabolism, exposure to sun and other environmental factors, toxins, cigarette smoke - all result in production of free radicals. The free radicals cause a dangerous chain reaction that destroys cellular compounds and can damage DNA, proteins and lipids (fats). When it comes to skin aging, free radicals interfere with collagen production and cause premature aging.



Fig. 3. Free radical scavenging activity A rapid, simple and inexpensive method to measure antioxidant capacity of Material involves the use of the free radical, 2,2-Diphenyl-1-picrylhydrazyl (DPPH).

Fig. 4. Superoxide anion scavenging activity The Superoxide anion scavenging activity was determined by a colormetric method (SOD assay kit; Dojindo Laboratories, Japan).

▶ Scaling (Corneocyte) Measurement (Tape Stripping Method)
Naturotics™ and a control product were applied on the forearms of volunteers twice/day for 7 consecutive days. Skin surface was analyzed by tape stripping method using a Corneofix® (Courage-Khazaka Electronic GmbH, Germany).



Fig. 5. Image taken with the special foil CORNEOFIX® F20. The different thickness of the corneocytes is shown in 5 different colors.

▶ Hydration Measurement
Naturotics™ and a control product were applied on the forearms of volunteers twice/day for 7 consecutive days. One area remained untreated. The water retention capacity of the skin was determined by measuring skin hydration using a corneometer (Courage-Khazaka Electronic GmbH, Germany).

Fig. 6. Skin hydration measured by corneometry. After the application was ceased, areas treated with the Control showed a rapid decrease in hydration. In contrast, Naturotics™ showed a significantly longer lasting hydration, up to 6 days after the last application (day 13) of 2 Naturotics™.

5. Safety & Toxicity

(1) Clinical safety Evaluation (Dermapro skin testing center)

Naturotics™ showed no significant evidence of primary irritation on human skin.

(2) Acute Oral Toxicity

The acute oral toxicity of a Natural Cosmetic containing 3% Naturotics™ was evaluated in rats. Twenty animals were fed 5,000mg/kg of the test substance. Neither mortality nor other abnormalities were observed.

6. Commercial Value

(1) The Press Release (November 3, 2005 [Consumer Safety Center] )

A. Antiseptic substance and bacteria found in non-antiseptic natural cosmetic products
Recently, the Korea Consumer Protection Board (KCPB) tested the contamination status of microorganism and the containment level of antiseptic substances in 10 types of natural cosmetic products sold in Internet shopping malls. Among them, 7 were found to contain antiseptics and 1 product contained bacteria. KCPB also tested the contamination level of microorganisms in 60 types of general cosmetic products.

B. Antiseptic substance detected in 70% of natural cosmetic products advertised as containing no antiseptics
All 10 natural cosmetic products were advertised as ‘non-antiseptic’, ‘artificial paraben or synthetic chemicals not used’ or ‘100% natural ingredients’. But antiseptic substances such as methyl paraben and phenoxy ethanol were found in 7 of the products.