1. Magnotics™

▶ Antimicrobial and anti inflammatory active agent for acne prone skin.
▶ Inhibitory effect against Propionibacterium sp. which are acne-causing bacteria.
▶ Natural antibiotic agent composed of 4 kinds of herbal extracts.
▶ Efficacy has proven by in vitro test and in vivo clinical trials.

2. Synthetic and Therapeutic Agents

As therapeutic agents for acne, antibiotics are usually employed to inhibit inflammation or kill the bacteria. For example, triclosan, benzyl peroxide, azelaic acid, retinoid, tetracycline, erythromycin, macrolide and clindamycin are among these antibiotics. However, these antibiotics have known to induce side effects. Benzyl peroxide and retinoid bring about xerosis cutis and skin irritation if they are used excessively as treatments and skin irritation. Tetracyclin, erythromycin, macrolide and clindamycin, generally used to medical treatment, have shown side effects such as appearance of resistant bacteria, organ damage, and immunohypersensitivity if they have been taken for a long time

Table. 1 Summary of synthetic agents against P.acne

In this way, scientific studies have shown that antibacterial substances related in acne-care products break down into biotoxins, which occasionally contaminate products during production and exposure to sunlight in the general environment.

3. Acne & Magnotics™

Acne, an inflammatory disease of the sebaceous glands, is a common skin disease that induces inflammation at the skin surface of the face, neck, chest, or back. Acne develops mostly in young people due to several factors, namely, hormonal imbalance, bacterial infection, stress, food, or cosmetic application. Especially, Propionibacterium acnes induces an inflammation in the sebaceous glands or hair pore. P. acnes secretes lipase and degrades sebum oils into free fatty acids, which are potent acne stimuli. Therefore, P. acnes is considered to play an important role in acne development by secreting inflammation-inducing factors.
Based on our natural compound technology, Magnotics™ has been proved to be very effective against tested on Propionibacterium sp. at a concentration as low 2% and we also have developed Magnotics™ as an effective natural complex for various applications in cosmetic field. Magnotics™ is an optimum synergistic combination of botanical fractions of 4 kinds of herbal extracts which are chosen and mixed in adequate proportions to give a high level of antiseptic activity.

4. The main components of Magnotics™

5. EXPERIMENTAL DATA

(1) Anti microbial activity of Magnotics™

▶ Disk diffusion susceptibility test

Fig 1. Photographic observation: Anti-microbial test with 2% of Magnotics on P. acnes.

Table 2. Measurement of clear zone size

a: Added amount of Magnotics™ per disc (%)
b: Average of three observations, Clear zone contains disk diameter.

In paper disk method, Fig 1 and Table 2 showed antimicrobial activities against the bacteria. The diameter of inhibitory zone was a linear function of logarithmic concentration in a range of 0.25%~2%/disk against two tested strains.

▶ MIC measurement

Anti microbial activity was also determined by MIC test against P. acnes. Magnotics™ showed the lowest MIC of 0.013% capable of inhibiting the organism.

Fig. 2. Photographic observation: MIC test on three strains

(2) ANTI-INFLAMMATORY ACTIVITY OF Magnotics™

It is widely accepted that the main proinflammatory mediators induced by bacteria and their cell components are cytokines, primarily TNF-α, and interleukin-8. Therefore, to investigate anti-inflammatory effects of Magnotics™, we performed ELISA for interleukin-8 and TNF-α in THP-1 cells. As shown in Fig. 3A and 3B, P. acnes-induced production of TNF-α, and interleukin-8 in THP-1 cells was all reduced by Magnotics™
Magnotics™ inhibited production of TNF-α and IL-8 as dose dependent manner up to 2% but not 5%. But in both cases of 5%, Magnotics™ did not inhibited cytokine production more potently than 2%. This result led us to guess that 2% of Magnotics™ is optimal concentration in case of interleukin-8.

7. AREAS OF COMMERCIAL VALUE

Magnotics™ is a powerful natural antibacterial and anti-inflammatory active agent. Magnotics™ would be a safe effective natural antibiotic for acne. Magnotics™ is stable and effective over the pH range typically found in personal care products. They also possess average thermal stability. It has value as an active ingredient in:

As a Natural Antibiotics:
* A few biggest korean cosmetic manufacturer are used this ingredient to replace a synthetic antimicrobial reagent, triclosan or benzyl peroxide. We are also providing atopy-care products used Magnotics™ as natural antibiotics in internet shopping mall. (www.skincure.co.kr)

(1) Recommended Use Level

All kinds of skin, body and hair care products including soaps, cleansers, lotion, gel, cream and so on.Usage levels may vary from 1% to 3%, depending on the formulation, the typical usage level averages around 2%.
* Do not use in food industry.

(2) How to Get the Most of Magnotics™

Do not add Magnotics™ to the formulation at temperature above 55℃, where there is a heating stage. It is recommended to add after the cooling process. If possible avoid UV or strong light.

8. Contact

BioSpectrum Inc.
101-701 SK Ventium, 522 Dangjeong-Dong, Gunpo-City, Gyeonggi-Do, 435-776, Rep. of Korea
Tel: +82-31-436-2090~3
Fax : +82-31-436-0605
E-Mail : kmh@biospectrum.com