Stem cell research

Stem cell study and application in BioSpectrum

Cell culture technology is used in virtually all fields of biomedical research and testing. We have developed a stem cell culture model for finding new compounds to activate stem cell proliferation and to protect stem cell inhibition. For example, stem cell activator like the effects of Transforming Growth Factor (TGF)-β1-elevating agents on the activity of TGF-β1 promoter are studied using the luciferase (LUC)-reporter-gene system.(Figure1)

Figure 1. Screening system with TGF-β1-Luc-reporter-gene system with stem cells

The cytokine TGF-β1 plays an important role in regulating the wound healing and tissue repair process; and aberration of its action has been implicated in several pathological processes including cancer, autoimmune disease and fibrosis. Therefore, TGF-β1 activators are very useful for skin care products and potentially become stem cell activators. In addition, activated stem cells in the skin will closely watch the epidermis, dermis and hair follicle in the skin searching for areas of the skin. Then activated stem cells proliferate and travel to these tissues to strengthen themselves.

Normally young stem cells are quickly regenerated, while old stem cells take much longer. Actually these cells are very weak, sometimes damaged or aged. Nonetheless system for stem cell division and movement into the basal layer of epidermis is functioning at optimal numbers. The older we get, the fewer stem cells are moved from hair follicle to the basal layer of epidermis. Less stem cell is divided into new stem cells, and we succumb to health problems, injury and aging more easily.

However, none of them is rebuilt in the body. None of them can actually bring back new tissue, bone, organs, or any part of the body. Recently BioSpectrum is attempt to be developing new ingredients to rebuilds the skin from own adult stem cells. Generally, traditional cosmetics nourish only adult cells and do not create new stem cells or activate stem cells. However, the innovative skin care products of BioSpectrum protect longevity and combats chronological aging while delaying senescence of skin cells by stem cell activation and proliferation. It also protects and preserves the youthful look and vitality of your skin.

Construction of 3D spheroid cultures at BioSpectrum

Our focus is the transition of cell cultures from conventional 2D adherent cultures to 3D spheroid cultures. Generally, 2D culture system results in errors in predicting tissue-specific responses due to the loss of native morphology and limited cell to cell and cell to matrix interactions. Thus, we are faced with development of alternative test methods in cosmetics. 3D spheroid cultures are physiologically similar to natural skin and can be used for the testing of drugs, cosmetics, irritancy, and toxicity studies as well as for wound healing models and cancer research. Additionally, to overcome several limitations of 2D adherent cultures, the development of 3D spheroid cultures has created cultures that can simulate tissue microenvironment and the improvement in 3D modeling is necessary to discriminate specific types of cell-to-cell or cell-to-matrix interactions. The main tasks of the BioSpectrum Life Science Institute (BLSI) has been developed new methods that can use a better screening process following a competitive advantage and a pre-clinical testing combined with an innovative scientific technology,replace. Also, the novel assay is helpful to reduce and refine the use of animals in scientific testing. In paricular, the data values produced by the 3D spheroid cultures, matrix culture, hanging drop culture, and combined with 3D skin culture and ADSC cells culture show new technological challenges, an advanced progress of the use of several 3D culture methods in cosmetic raw materials, the potential to mimick physiologically 3D skin-like tissues, and successfully available protocols for culturing and analysis. (Figure 2)

We developed a new reporter gene assay expressing luciferase(Luc) under the control of the microphthalmia-associated transcription factor (MITF) in stem cell. MITF is the master regulator of the pigmentation. This assay was highly sensitive and reproducible, and responded as expected to melanogenesis regulators such as Forskolin, α-melanin stimulating hormone(α-MSH), etc. The transfected cells were increased the expression of luciferase in response to melanogenesis signal or down regulated with known melanogenesis inhibitors.